Cerberus

The 3-headed feral gatekeeper. Said to have the power to control ice. He grants power to those worthy of it. Those unworthy, meet their doom by his giant fangs.

First appearance: Mission 3
Strategy: Refer to boss strategies.

Agni

One of the Twin Demon gatekeepers. Agni has the power to control fire. In essence a sword being swung by a demon host. Searching for an owner worthy of its full power.

First appearance: Mission 5
Strategy: Refer to boss strategies.

Rudra

One of the Twin Demon gatekeepers. Rudra has the power to control wind. In essence a sword being swung by a demon host. Searching for an owner worthy of its full power.

First appearance: Mission 5
Strategy: Refer to boss strategies.

Vergil

Dante's half-man, half-demon twin brother. Wields a keepsake sword from his father but won't touch guns as he doesn't view them as weapons of a true warrior.

First appearance: Mission 7
Strategy: Refer to boss strategies.

Heart of Leviathan

Heart of the great beast Leviathan. The heart itself should be considered its own entity. One that will protect itself as all costs.

First appearance: Mission 8
Strategy: Refer to boss strategies.

Nevan

One of the demon gatekeepers sealed away by Sparda. She seduces humans down the dark path to hell. Attacks foes with a combination of electricity and bats she controls.

First appearance: Mission 9
Strategy: Refer to boss strategies.

Beowulf

Gatekeeper sealed away by Sparda for 2000 years. He anxiously awaits his chance at revenge. His overwhelming strength and light attacks make him a force to fear.

First appearance: Mission 11
Strategy: Refer to boss strategies.

Geryon

Once a horse of legend ridden by countless heroes, ingesting demon soul essence darkened his once bright soul. Has the ability to control time on a limited scale

First appearance: Mission 12
Strategy: Refer to boss strategies.

Vergil 2

Absorbing Beowulf's light power has given birth to a new stronger Vergil. Now in addition to Vergil's amazing sword skill, he has mixed in some new martial art moves.

First appearance: Mission 13
Strategy: Refer to boss strategies.

Lady

Female devil hunter that seems to block Dante's every move. With little training and even less power, her most helpful weapon is her belief that what she is doing is right.

First appearance: Mission 16
Strategy: Refer to boss strategies.

Doppelganger

Demon that takes the same shape of his foes. His ability is produced by refracting light in various directions. Due to this, he is essentially a demon composed of shadow.

First appearance: Mission 17
Strategy: Refer to boss strategies.

Arkham

Transformed into a gruesome beast after obtaining Sparda's power. His form was not born of Sparda's inherited power, but rather from the darkness that lies deep in his heart.

First appearance: Mission 19
Strategy: Refer to boss strategies.

Arkham's Legions

A swarm of demons generated from Arkham's body. These non-intelligent demons serve one purpose: to damage foes by exploding on impact with them.

First appearance: Mission 19
Strategy: Refer to boss strategies under Arkham.

Vergil 3

After obtaining the Force Edge, the symbol of Sparda, Vergil is complete. The only way to compete with such an awesome foe is to believe in oneself and one's weapons.

First appearance: Mission 20
Strategy: Refer to boss strategies.

Jester

Mysterious clown that appears and challenges Dante. His motives are unknown. Gestures resemble those of a circus ringmaster when he casts his magic.

First appearance: Mission 5 (DMC3 special edition only)
Strategy: Refer to boss strategies.